A Secret Weapon For thr77
A Secret Weapon For thr77
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in many tumors, which results in chromosome instability, and therefore may possibly lead to tumorigenesis. This protein was observed to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in advertising cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two more splice variants ended up noted but in depth nucleotide sequence details is not nonetheless readily available.
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Blocking peptides are peptides that bind exclusively to your concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.
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This manual outlines even more possible optimization of cellular and immunoassay parameters to guarantee the very best success are...
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This guidebook outlines even further probable optimization of mobile and immunoassay parameters to be click here certain the very best results are...
Antibodies bound to the blocking peptide now not bind to the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is specific.
Target Information and facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose associates are characterized by a spectacular periodicity in protein abundance from the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinctive expression and degradation patterns which lead for the temporal coordination of each and every mitotic function. Cyclin E forms a fancy with and functions being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates with the G1-S phase boundary and is also degraded as cells development via S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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